James W. Clancy Research Assistant Professor

Biology and biogenesis of tumor-derived extracellular vesicles
James W.  Clancy

Research Interests:

All cells release a wide array of membrane-encapsulated vesicles into the surrounding environment. Tumor cells are capable of co-opting this process resulting in the shedding of extracellular vesicles, known as tumor-derived microvesicles (TMVs), which are released directly from the surface of invading tumor cells. Upon release, TMVs are now understood to serve multiple pro-tumorigenic roles including facilitating cell invasion and serving as a platform for cell-cell communication. While much has been learned about the regulatory networks controlling their release, our understanding of cargo trafficking and the influence of GTPase signaling remain active fields of investigation. My on-going research interests center on understanding the biogenesis and functional consequences stemming from the shedding of TMVs. Specifically, I am interested in the trafficking of nucleic acid cargo to sites of active TMV biogenesis at the cell periphery, and the pathophysiological impacts of cargo transfer to recipient cells.

 

Biography:

  • Research Assistant Professor, Department of Biological Sciences, University of Notre Dame, IN 2020-present
  • Postdoctoral Research Associate, Department of Biological Sciences, University of Notre Dame, IN 2014-2020
  • Ph.D., University of Notre Dame, IN 2014
  • B.S. Bioengineering, University of California, San Diego, CA 2005

 

Recent Papers:

  • J. Clancy, Y. Zhang, C. Sheehan, and C. D'Souza‐Schorey. "An ARF6-Exportin-5 Axis Delivers Pre-miRNA Cargo to Tumor Microvesicles."  Nat Cell Biol. 21, 856-866, 2019.
  • J. Clancy, C. Tricarico, D. Marous, and C. D'Souza‐Schorey. "Coordinated Regulation of Intracellular Fascin Distribution Governs Tumor Microvesicle Release and Invasive Cell Capacity." Mol Cell Biol. 39: e00264-18, 2019.
  • J. Clancy, A. Sedgwick, C. Rosse, M. Method, P. Chavrier, and C. D’Souza-Schorey. “Regulated delivery of molecular cargo to invasive tumor-derived microvesicles.” Nat. Comm. 6:6919, 2015.
  • A. Sedgwick, J. Clancy, M. O. Balmert, and C. D’Souza- Schorey. “Extracellular Microvesicles and Invadopodia Mediate Non-overlapping Modes of Tumor Cell Invasion” Nat. Sci. Reports. 5:14748, 2015.
  • V. Muralidharan-Chari, J. Clancy, C. Plou, M. Romao, P. Chavrier, G. Raposo, and C. D'Souza-Schorey. “ARF6-Regulated Shedding of Tumor Cell-Derived Plasma Membrane Microvesicles.” Curr. Biol. 19(22): 1875-1885, 2009.