Manuela Lahne Research Assistant Professor
My research investigates the mechanisms that lead to robust regeneration of retinal neurons following damage of the adult zebrafish retina. I specifically focus on a migratory behavior termed interkinetic nuclear migration that the proliferating cells in the retina, the Müller glia and the subsequently arising neuronal progenitor cells, undergo. During retinal development this migration exposes the nuclei of proliferating cells to different signaling gradients that are critical in determining whether the proliferating cells remain in the cell cycle or differentiate into the different neuronal subtypes of the retina. In the adult regenerating retina, interkinetic nuclear migration plays a similar role; however the signaling gradients have not been identified. Currently, I am investigating both the mechanisms that facilitate interkinetic nuclear migration and those that influence fate decisions of proliferating cells. My research uses a variety of techniques including live cell imaging and molecular biological methods. Understanding the mechanisms that orchestrate the regenerative response in the zebrafish retina will benefit our long-term aim to develop strategies that will overcome vision impairments in human.
- Research Assistant Professor, University of Notre Dame 2015 - Present
- Postdoctoral Research Assistant, University of Notre Dame 2009 – 2015
- Postdoctoral Research Assistant, Queen Mary College, University of London, UK 2008 - 2009
- Ph.D. in Physiology, University College London, UK 2007
- Diploma in Biology, Humboldt-University, Berlin, Germany 2002
- Lahne M, Hyde DR. Live cell imaging – new avenues to investigate retinal regeneration. Neural Regeneration Research, 2017, 12(8), 1210-1219.
- Gorsuch R, Lahne M, Yarka C, Petravick M, Li J, Hyde D.R. Sox2 regulates Müller glia reprogramming and proliferation in the regenerating zebrafish retina via Lin28 and Ascl1a. Exp Eye Res, 2017, 161, 174-12.
- Lahne M, Gorsuch RA, Nelson CM, Hyde DR. Culture of adult transgenic zebrafish retinal explants for live cell imaging by multiphoton microscopy. J Vis Exp, 2017, 120 (doi:10.3791/55335).
- Lahne M, Li J, Marton RM, Hyde DR. Actin cytoskeleton and Rock-mediated INM are required for photoreceptor regeneration in the adult zebrafish retina. Journal of Neuroscience, in press.
- Lahne M, Hyde DR. Interkinetic nuclear migration in the adult regenerating retina. Adv. Exp. Med. Bio, 2016, 854, 587- 593.
- Conner C, Ackerman KM, Lahne M, Hobgood JS, Hyde DR. TNFα expression and repressing Notch signaling are sufficient to mimic retinal regeneration by inducing Müller glial proliferation to generate committed progenitor cells. Journal of Neuroscience, 2014, 34(43), 14403-14419.
- Lahne M, Gale JE. Damage-induced cell–cell communication in different cochlear cell types via two distinct ATP-dependent Ca2+ waves. Purinergic Signalling, 2010, 6 (2), 189-200.
- Lahne M, Gale JE. Damage-induced activation of ERK1/2 in cochlear supporting cells is a hair cell death promoting signal that depends upon extracellular ATP and calcium. Journal of Neuroscience, 2008, 28 (19), 4918-4928.